Device

Part:BBa_K318014

Designed by: Nathaniel Pantalone, Justin Vrana   Group: iGEM10_Wisconsin-Madison   (2010-07-13)


pCons + attB + RBS + GFP + T + rRFP + rRBS + attP

This composite part is a testing cassette for a Cre-like, P22 Int recombination. The constitutive promoter should express GFP if recombination does not occur. If Cre-like recombination occurs, this part should express RFP. It is composed of the following biobricks: BBa_J23100, BBa_I11032, BBa_E0240, BBa_K199021, BBa_I11033.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 969
    Illegal AgeI site found at 1081
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 741


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Categories
Parameters
None